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簡要描述：腺苷受體蛋白抗體免 疫 原；KLH conjugated synthetic peptide derived from cAMP-receptor protein: 2-100/210
產(chǎn)品型號：GOY-01K0246廠商性質(zhì)：科研更新時(shí)間：2023-10-24 00:00:00訪 問 量：144產(chǎn)品分類
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腺苷受體蛋白抗體
產(chǎn)品名稱 | 英文名稱 | 貨號 |
腺苷受體蛋白抗體 | cAMP-receptor protein | GOY-01K0246 |
商品屬性：
英文名稱: cAMP-receptor protein
中文名:腺苷受體蛋白抗體
別 名；CRP_ECOLI;
cAMP-activated global transcriptional regulator CRP; Catabolite activator
protein; CAP; Catabolite gene activator; cAMP receptor protein; CRP; cAMP
regulatory protein.
研究領(lǐng)域；細(xì)胞生物 免疫學(xué) 轉(zhuǎn)錄調(diào)節(jié)因子 細(xì)胞膜受體
抗體來源；Rabbit
克隆類型；Polyclonal
產(chǎn)品應(yīng)用；WB=1:500-2000
ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)）
not yet tested in
other applications.
optimal
dilutions/concentrations should be determined by the end user.
理論分子量；24kDa
細(xì)胞定位；細(xì)胞膜
性 狀；Liquid
濃 度；1mg/ml
免 疫 原；KLH
conjugated synthetic peptide derived from cAMP-receptor protein: 2-100/210
亞 型；IgG
純化方法；affinity
purified by Protein A
緩 沖 液；0.01M
TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
注意事項(xiàng)；This
product as supplied is intended for research use only, not for use in human,
therapeutic or diagnostic applications.
產(chǎn)品介紹:
Escherichia coli contains more than 250 known
promoters that must be activated prior to transcription. cAMP receptor
protein (crp), also known as cAMP regulatory protein and catabolite gene
activator, is a 210 amino acid E. coli protein that complexes with cyclic AMP
and binds to specific DNA sites near the promoter, resulting in homodimeric
transcription activation of more than 100 E. coli promoters. Acting as a
negative regulator against its own synthesis, crp regulates transcription of
several catabolite-sensitive operons. Crp activates transcription by making
multiple interactions with RNA polymerase. Containing one cyclic nucleotide
binding domain and one HTH crp-type DNA binding domain, crp binds DNA as a
dimer to induce a severe bend in the DNA. This formation is a regulatory
mechanism of crp gene expression that plays a major role in determining the
molecular mechanism in gene transcription. A global transcription regulator. Complexes with
cyclic AMP (cAMP) which allosterically activates DNA binding (to consensus
sequence 5'-AAATGTGATCTAGATCACATTT-3') to directly regulate the transcription
of about 300 genes in about 200 operons and indirectly regulate the
expression of about half the genome. There are 3 classes of CRP promoters;
class I promoters have a single CRP-binding site upstream of the RNA
polymerase (RNAP)-binding site, whereas in class II promoters the single CRP-
and RNAP-binding site overlap, CRP making multiple contacts with RNAP. Class
III promoters require multiple activator molecules, including at least one
CRP dimer. It can act as an activator, repressor, coactivator or corepressor.
Induces a severe bend in DNA (about 87 degrees), bringing upstream promoter
elements into contact with RNAP. Acts as a negative regulator of its own
synthesis as well as for adenylate cyclase (cyaA), which generates cAMP. High
levels of active CRP are detrimental to growth (PubMed:16260780). Plays a
major role in carbon catabolite repression (CCR). CCR involves cAMP,
adenylate cyclase (cyaA), CRP and the EIIA-Glc component of the PTS (crr). In
the presence of glucose EIIA-Glc is dephosphorylated, and does not activate
adenylate cyclase, leading to reduced cAMP and thus decreased CRP activity.
Also plays a role in many other processes (see PubMed:22573269). |
多克隆抗體制備：
我公司合成抗原，保證陽性血清和抗體ELISA效價(jià)達(dá)到1：32000，由蛋白抗原制備的多抗，保證WB檢測結(jié)果是陽性。技術(shù)服務(wù)實(shí)行分步報(bào)價(jià)，按節(jié)點(diǎn)收費(fèi)，為客戶節(jié)約成本。
鼠單克隆抗體制備:
客戶可根據(jù)項(xiàng)目實(shí)際需求情況靈活選擇進(jìn)行，我公司將為您的項(xiàng)目提供專業(yè)的建議。在制備過程中，與客戶互動(dòng)驗(yàn)證，確保最終制備的抗體ELISA陽性血清檢測達(dá)到合同要求的同時(shí)，也能滿足客戶抗體最終應(yīng)用驗(yàn)證需求。技術(shù)服務(wù)實(shí)行分步報(bào)價(jià)，按節(jié)點(diǎn)收費(fèi)，為客戶節(jié)約成本。
重組抗體表達(dá)服務(wù):
重組抗體技術(shù)使制備人源化抗體和人源抗體成為可能，而這是其他常規(guī)的多克隆或單克隆抗體制備方法無法做到的。我公司已搭建好的抗體測序、重組抗體表達(dá)等先進(jìn)平臺，能為客戶提供各種類型的重組抗體表達(dá)服務(wù)，包含鼠源單克隆抗體構(gòu)建及表達(dá)、更換恒定區(qū)亞型的嵌合抗體表達(dá)，抗體人源化改造及表達(dá)等服務(wù)。
單項(xiàng)免疫學(xué)及檢測服務(wù):
我公司也為客戶提供單項(xiàng)免疫學(xué)和檢測服務(wù)，如動(dòng)物免疫、動(dòng)物飼養(yǎng)、抗體修飾服務(wù)（如FITC，HRP等的標(biāo)記）、以及ELISA，WB，IHC，DB等檢測服務(wù)。
抗體定制服務(wù):
已注冊商標(biāo)，主要用于抗體藥物發(fā)現(xiàn)和開發(fā)，是我公司針對有特定應(yīng)用要求和各種模型測試的客戶量身定制的專業(yè)服務(wù)，主要面向工業(yè)客戶。
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