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簡要描述：p21激活激酶2抗體注意事項；This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產品型號：GOY-01K1948廠商性質：科研更新時間：2023-10-24 00:00:00訪 問 量：169產品分類
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商品屬性：
貨號 | 產品名稱 | 規(guī)格 |
GOY-01K1948 | p21激活激酶2抗體 | 50ul |
GOY-01K1948 | p21激活激酶2抗體 | 100ul |
GOY-01K1948 | p21激活激酶2抗體 | 200ul |
英文名稱: PAK2
中文名稱: p21激活激酶2抗體
別 名；C-t-PAK2; CB422; EC 2.7.11.1; Gamma PAK;
Gamma-PAK; hPAK65; Kinase; p21 (CDKN1A) activated kinase 2; p21
(CDKN1A)-activated kinase 2a; p21 activated kinase 2; p21 protein
(Cdc42/Rac)-activated kinase 2; p21 protein Cdc42 Rac activated kinase 2;
p21-activated kinase 2; p21-activated kinase, 65-KD; p21-activated protein kinase
I; p21CDKN1A activated kinase 2; p34; PAK 2; PAK-2; PAK-2p34; PAK2_HUMAN;
PAKgamma; S6 H4 kinase; S6/H4 kinase; Serine threonine protein kinase PAK 2;
Serine/threonine protein kinase PAK 2;
研究領域；腫瘤 免疫學 信號轉導 細胞凋亡 轉錄調節(jié)因子 激酶和磷酸酶
抗體來源；Rabbit
克隆類型；Polyclonal
交叉反應； Human, (predicted: Mouse, Rat, Rabbit, )
產品應用；WB=1:500-2000
ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復）
not yet tested in
other applications.
optimal
dilutions/concentrations should be determined by the end user.
理論分子量；58kDa
細胞定位；細胞核 細胞漿 細胞膜
性 狀；Liquid
濃 度；1mg/ml
免 疫 原；KLH conjugated synthetic
peptide derived from human PAK2: 131-230/524
亞 型；IgG
純化方法；affinity purified by Protein A
緩 沖 液；0.01M TBS(pH7.4) with 1% BSA,
0.03% Proclin300 and 50% Glycerol.
注意事項；This product as supplied is
intended for research use only, not for use in human, therapeutic or diagnostic
applications.
產品介紹 The p21 activated kinases
(PAK) are critical effectors that link Rho GTPases to cytoskeleton
reorganization and nuclear signaling. The PAK proteins are a family of
serine/threonine kinases that serve as targets for the small GTP binding
proteins, CDC42 and RAC1, and have been implicated in a wide range of
biological activities. The protein encoded by this gene is activated by
proteolytic cleavage during caspase mediated apoptosis, PAK2 also interacts
with and is activated by HIV1 Nef.
Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell motility, cell cycle progression, apoptosis or proliferation. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Full-length PAK2 stimulates cell survival and cell growth. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Phosphorylates JUN and plays an important role in EGF-induced cell proliferation. Phosphorylates many other substrates including histone H4 to promote assembly of H3.3 and H4 into nucleosomes, BAD, ribosomal protein S6, or MBP. Additionally, associates with ARHGEF7 and GIT1 to perform kinase-independent functions such as spindle orientation control during mitosis. On the other hand, apoptotic stimuli such as DNA damage lead to caspase-mediated cleavage of PAK2, generating PAK-2p34, an active p34 fragment that translocates to the nucleus and promotes cellular apoptosis involving the JNK signaling pathway. Caspase-activated PAK2 phosphorylates MKNK1 and reduces cellular translation.
實驗流程：
（1）特異性結合抗原：抗體本身不能直接溶解或殺傷帶有特異抗原的靶細胞，通常需要補體或吞噬細胞等共同發(fā)揮效應以清除病原微生物或導致病理損傷。然而，抗體可通過與病毒或毒素的特異性結合，直接發(fā)揮中和病毒的作用。
（2）活補體：IgM、IgG1、IgG2和IgG3可通過經典途徑激活補體，凝聚的IgA、IgG4和IgE可通過替代途徑激活補體。
（3）結合細胞：不同類別的免疫球蛋白，可結合不同種的細胞，參與免疫應答。
（4）可通過胎盤及粘膜：免疫球蛋白G（IgG）能通過胎盤進入胎兒血流中，使胎兒形成自然被動
免疫。免疫球蛋白A（IgA）可通過消化道及呼吸道粘膜，是粘膜局部抗感染免疫的主要因素。
（5）具有抗原性：抗體分子是一種蛋白質，也具有刺機體產生免疫應答的性能。不同的免疫球蛋白分子，各具有不同的抗原性。
（6）抗體對理化因子的抵抗力與一般球蛋白相同：不耐熱，60～70℃即被破壞。各種酶及能使蛋白質凝固變性的物質，均能破壞抗體的作用?？贵w可被中性鹽類沉淀。在生產上?？捎昧蛩徜@或硫酸鈉從免疫血清中沉淀出含有抗體的球蛋白，再經透析法將其純化。
抗體的制備過程：
1.免疫原：普通的大分子蛋白，通過分子克隆構建載體并在大腸桿菌中進行誘導表達獲得重組蛋白，純化鑒定后可直接作為免疫原；小分子蛋白或化合物等分子量小，需要偶聯(lián)載體對該分子進行改造才能使其成為具有免疫原性的抗原，常見偶聯(lián)載體如BSA、OVA、HAS等。
2. 免疫動物：常用于制備抗血清的動物有豚鼠、家兔、雞、大小鼠等，大量生產時需要用到狗、綿羊、山羊等。
3.免疫血清的收集：一般家兔、綿羊、山羊可采用靜動脈采血，家兔、豚鼠、大鼠、雞可采用心臟采血，家兔、山羊、綿羊可采用靜脈采血。
4. 免疫血清的純化與鑒定：得到的抗血清需要進一步的純化，利用偶聯(lián)了抗原的親和柱進行層析，具有高效，特異性強，純度高的特定。接著要鑒定純化蛋白的含量、相對分子的質量、純度以及特異性。
5.p21激活激酶2抗體免疫血清的保存：抗體一般比較穩(wěn)定，在-80℃ ～-20 ℃可以保存約5年而不會影響效價，而真空干燥保存時間可以更久。保存前需經除菌并添加防腐劑。
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