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簡要描述：血藍(lán)蛋白抗體免 疫 原；KLH protein: full length
產(chǎn)品型號：GOY-01K0105廠商性質(zhì)：科研更新時間：2023-10-24 00:00:00訪 問 量：219產(chǎn)品分類
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血藍(lán)蛋白抗體
產(chǎn)品名稱 | 英文名稱 | 貨號 |
血藍(lán)蛋白抗體 | KLH | GOY-01K0105 |
商品屬性：
英文名稱: KLH
中文名:血藍(lán)蛋白抗體
別 名；Keyhole limpet
hemocyanin (KLH); KLH 1 ; KLH1; Keyhole limpet hemocyanin A; KLH-A; Hemocyanin
1; hemocyanin A chain.
研究領(lǐng)域；免疫學(xué)
抗體來源；Mouse
克隆類型；Polyclonal
產(chǎn)品應(yīng)用；WB=1:500-2000
ELISA=1:5000-10000
not yet tested in
other applications.
optimal
dilutions/concentrations should be determined by the end user.
理論分子量；800-900kDa
性 狀；Liquid
濃 度；1mg/ml
免 疫 原；KLH
protein: full length
亞 型；IgG
純化方法；affinity
purified by Protein A
緩 沖 液；0.01M
TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件；Shipped at
4℃. Store at -20 °C for one
year. Avoid repeated freeze/thaw cycles.
產(chǎn)品介紹:
Keyhole limpet hemocyanin is an extremely large,
heterogeneous glycosylated protein consisting of subunits with a molecular
weight of 350,000 and 390,000 in aggregates with molecular weights of
4,500,000-13,000,000. Each domain of a KLH subunit contains two copper atoms
that together bind a single oxygen molecule (O2). When oxygen is bound to
hemocyanin, the molecule takes on a distinctive transparent, opalescent blue
color. The KLH protein is potently immunogenic yet safe in humans and is therefore
highly prized as a vaccine carrier protein. The large and highly glycosylated
KLH protein cannot be reproduced synthetically. It is available only as a
purified biological product from the Keyhole Limpet Megathura crenulata. Keyhole limpet hemocyanin (KLH) is used
extensively as a carrier protein in the production of antibodies for
research, biotechnology and therapeutic applications. Haptens are substances
with a low molecular weight such as peptides, small proteins and drug
molecules that are generally not immunogenic and require the aid of a carrier
protein to stimulate a response from the immune system in the form of
antibody production.[2] KLH is the most widely employed carrier proteins for
this purpose. KLH is an effective carrier protein for several reasons. Its
large size and numerous epitopes generate a substantial immune response, and
abundance of lysine residues for coupling haptens, allows a high
hapten:carrier protein ratio increasing the likelihood of generating
hapten-specific antibodies. In addition, because KLH is derived from the
limpet, a gastropod, it is phylogenetically distant from mammalian proteins,
thus reducing false positives in immunologically based research techniques in
mammalian model organisms. KLH may also be a challenging molecule to work
with because of its propensity to aggregate and precipitate. Aggregates
remain immunogenic, but limit the ability to conjugate haptens and are
difficult to manipulate in the laboratory. A high quality KLH preparation
with the clear opalescent blue color is the best indicator of KLH solubility. |
多克隆抗體制備：
我公司合成抗原，保證陽性血清和抗體ELISA效價達(dá)到1：32000，由蛋白抗原制備的多抗，保證WB檢測結(jié)果是陽性。技術(shù)服務(wù)實(shí)行分步報(bào)價，按節(jié)點(diǎn)收費(fèi)，為客戶節(jié)約成本。
鼠單克隆抗體制備:
客戶可根據(jù)項(xiàng)目實(shí)際需求情況靈活選擇進(jìn)行，我公司將為您的項(xiàng)目提供專業(yè)的建議。在制備過程中，與客戶互動驗(yàn)證，確保最終制備的抗體ELISA陽性血清檢測達(dá)到合同要求的同時，也能滿足客戶抗體最終應(yīng)用驗(yàn)證需求。技術(shù)服務(wù)實(shí)行分步報(bào)價，按節(jié)點(diǎn)收費(fèi)，為客戶節(jié)約成本。
重組抗體表達(dá)服務(wù):
重組抗體技術(shù)使制備人源化抗體和人源抗體成為可能，而這是其他常規(guī)的多克隆或單克隆抗體制備方法無法做到的。我公司已搭建好的抗體測序、重組抗體表達(dá)等先進(jìn)平臺，能為客戶提供各種類型的重組抗體表達(dá)服務(wù)，包含鼠源單克隆抗體構(gòu)建及表達(dá)、更換恒定區(qū)亞型的嵌合抗體表達(dá)，抗體人源化改造及表達(dá)等服務(wù)。
單項(xiàng)免疫學(xué)及檢測服務(wù):
我公司也為客戶提供單項(xiàng)免疫學(xué)和檢測服務(wù)，如動物免疫、動物飼養(yǎng)、抗體修飾服務(wù)（如FITC，HRP等的標(biāo)記）、以及ELISA，WB，IHC，DB等檢測服務(wù)。
抗體定制服務(wù):
已注冊商標(biāo)，主要用于抗體藥物發(fā)現(xiàn)和開發(fā)，是我公司針對有特定應(yīng)用要求和各種模型測試的客戶量身定制的專業(yè)服務(wù)，主要面向工業(yè)客戶。
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