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簡(jiǎn)要描述：丙型肝炎病毒NS5B抗體免 疫 原；KLH conjugated synthetic peptide derived from Hepatitis C virus NS5B.: 21-102/102
產(chǎn)品型號(hào)：GOY-01K4598廠商性質(zhì)：科研更新時(shí)間：2023-10-24 00:00:00訪 問(wèn) 量：139產(chǎn)品分類(lèi)
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商品屬性：
貨號(hào) | 產(chǎn)品名稱(chēng) | 規(guī)格 |
GOY-01K4598 | 丙型肝炎病毒NS5B抗體 | 50ul |
GOY-01K4598 | 丙型肝炎病毒NS5B抗體 | 100ul |
GOY-01K4598 | 丙型肝炎病毒NS5B抗體 | 200ul |
英文名稱(chēng): Hepatitis C virus NS5B
中文名稱(chēng): 丙型肝炎病毒NS5B抗體
別 名；HCV NS5B; Hepatitis C virus NS5B; Non
structural protein 5B; p68; RNA dependent RNA polymerase; RNA directed RNA
polymerase; Hepatitis C virus; Hepatitis C virus subtype 1a; Hepatitis C virus
subtype 2a; Hepatitis C virus subtype 1b; Hepatitis C virus subtype 2c;
Hepatitis C virus subtype 3a; Hepatitis C virus genotype 2; Hepatitis C virus
subtype 6a.
研究領(lǐng)域；微生物學(xué) 細(xì)菌及病毒
抗體來(lái)源；Rabbit
克隆類(lèi)型；Polyclonal
產(chǎn)品應(yīng)用；ELISA=1:5000-10000
not yet tested in
other applications.
optimal
dilutions/concentrations should be determined by the end user.
理論分子量；11kDa
性 狀；Liquid
濃 度；1mg/ml
免 疫 原；KLH conjugated synthetic
peptide derived from Hepatitis C virus NS5B.: 21-102/102
亞 型；IgG
純化方法；affinity purified by Protein A
緩 沖 液；0.01M TBS(pH7.4) with 1% BSA,
0.03% Proclin300 and 50% Glycerol.
保存條件；Shipped at 4℃. Store at -20 °C for one year. Avoid
repeated freeze/thaw cycles.
注意事項(xiàng)；This product as supplied is
intended for research use only, not for use in human, therapeutic or diagnostic
applications.
Function:
Core protein
packages viral RNA to form a viral nucleocapsid, and promotes virion budding.
Modulates viral translation initiation by interacting with HCV IRES and 40S ribosomal
subunit. Also regulates many host cellular functions such as signaling pathways
and apoptosis. Prevents the establishment of cellular antiviral state by
blocking the interferon-alpha/beta (IFN-alpha/beta) and IFN-gamma signaling
pathways and by inducing human STAT1 degradation. Thought to play a role in
virus-mediated cell transformation leading to hepatocellular carcinomas.
Interacts with, and activates STAT3 leading to cellular transformation. May
repress the promoter of p53, and sequester CREB3 and SP110 isoform 3/Sp110b in
the cytoplasm. Also represses cell cycle negative regulating factor CDKN1A,
thereby interrupting an important check point of normal cell cycle regulation.
Targets transcription factors involved in the regulation of inflammatory
responses and in the immune response: suppresses NK-kappaB activation, and
activates AP-1. Could mediate apoptotic pathways through association with
TNF-type receptors TNFRSF1A and LTBR, although its effect on death
receptor-induced apoptosis remains controversial. Enhances TRAIL mediated
apoptosis, suggesting that it might play a role in immune-mediated liver cell
injury. Seric core protein is able to bind C1QR1 at the T-cell surface,
resulting in down-regulation of T-lymphocytes proliferation. May transactivate
human MYC, Rous sarcoma virus LTR, and SV40 promoters. May suppress the human
FOS and HIV-1 LTR activity. Alters lipid metabolism by interacting with
hepatocellular proteins involved in lipid accumulation and storage. Core
protein induces up-regulation of FAS promoter activity, and thereby probably
contributes to the increased triglyceride accumulation in hepatocytes
(steatosis).
實(shí)驗(yàn)流程：
（1）特異性結(jié)合抗原：抗體本身不能直接溶解或殺傷帶有特異抗原的靶細(xì)胞，通常需要補(bǔ)體或吞噬細(xì)胞等共同發(fā)揮效應(yīng)以清除病原微生物或?qū)е虏±頁(yè)p傷。然而，抗體可通過(guò)與病毒或毒素的特異性結(jié)合，直接發(fā)揮中和病毒的作用。
（2）活補(bǔ)體：IgM、IgG1、IgG2和IgG3可通過(guò)經(jīng)典途徑激活補(bǔ)體，凝聚的IgA、IgG4和IgE可通過(guò)替代途徑激活補(bǔ)體。
（3）結(jié)合細(xì)胞：不同類(lèi)別的免疫球蛋白，可結(jié)合不同種的細(xì)胞，參與免疫應(yīng)答。
（4）可通過(guò)胎盤(pán)及粘膜：免疫球蛋白G（IgG）能通過(guò)胎盤(pán)進(jìn)入胎兒血流中，使胎兒形成自然被動(dòng)
免疫。免疫球蛋白A（IgA）可通過(guò)消化道及呼吸道粘膜，是粘膜局部抗感染免疫的主要因素。
（5）具有抗原性：抗體分子是一種蛋白質(zhì)，也具有刺機(jī)體產(chǎn)生免疫應(yīng)答的性能。不同的免疫球蛋白分子，各具有不同的抗原性。
（6）抗體對(duì)理化因子的抵抗力與一般球蛋白相同：不耐熱，60～70℃即被破壞。各種酶及能使蛋白質(zhì)凝固變性的物質(zhì)，均能破壞抗體的作用?？贵w可被中性鹽類(lèi)沉淀。在生產(chǎn)上?？捎昧蛩徜@或硫酸鈉從免疫血清中沉淀出含有抗體的球蛋白，再經(jīng)透析法將其純化。
抗體的制備過(guò)程：
1.丙型肝炎病毒NS5B抗體免疫原：普通的大分子蛋白，通過(guò)分子克隆構(gòu)建載體并在大腸桿菌中進(jìn)行誘導(dǎo)表達(dá)獲得重組蛋白，純化鑒定后可直接作為免疫原；小分子蛋白或化合物等分子量小，需要偶聯(lián)載體對(duì)該分子進(jìn)行改造才能使其成為具有免疫原性的抗原，常見(jiàn)偶聯(lián)載體如BSA、OVA、HAS等。
2. 免疫動(dòng)物：常用于制備抗血清的動(dòng)物有豚鼠、家兔、雞、大小鼠等，大量生產(chǎn)時(shí)需要用到狗、綿羊、山羊等。
3. 免疫血清的收集：一般家兔、綿羊、山羊可采用靜動(dòng)脈采血，家兔、豚鼠、大鼠、雞可采用心臟采血，家兔、山羊、綿羊可采用靜脈采血。
4. 免疫血清的純化與鑒定：得到的抗血清需要進(jìn)一步的純化，利用偶聯(lián)了抗原的親和柱進(jìn)行層析，具有高效，特異性強(qiáng)，純度高的特定。接著要鑒定純化蛋白的含量、相對(duì)分子的質(zhì)量、純度以及特異性。
5. 免疫血清的保存：抗體一般比較穩(wěn)定，在-80℃ ～-20 ℃可以保存約5年而不會(huì)影響效價(jià)，而真空干燥保存時(shí)間可以更久。保存前需經(jīng)除菌并添加防腐劑。
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