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簡(jiǎn)要描述：人類(lèi)免疫缺陷病毒2型/2型艾滋病病毒gp36抗體注意事項(xiàng)；This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品型號(hào)：GOY-01K0961廠商性質(zhì)：科研更新時(shí)間：2023-10-24 00:00:00訪 問(wèn) 量：139產(chǎn)品分類(lèi)
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商品屬性：
貨號(hào) | 產(chǎn)品名稱(chēng) | 規(guī)格 |
GOY-01K0961 | 人類(lèi)免疫缺陷病毒2型/2型艾滋病病毒gp36抗體 | 50ul |
GOY-01K0961 | 人類(lèi)免疫缺陷病毒2型/2型艾滋病病毒gp36抗體 | 100ul |
GOY-01K0961 | 人類(lèi)免疫缺陷病毒2型/2型艾滋病病毒gp36抗體 | 200ul |
英文名稱(chēng): HIV2 gp36
中文名稱(chēng): 人類(lèi)免疫缺陷病毒2型/2型艾滋病病毒gp36抗體
別 名；Gp36; HIV-2 gp36; HIV2 gp36; HIV2gp36; gp36;
HIV 2; Human immunodeficiency virus 2; Human Immunodeficiency Virus Type 2;
ENV_HV2RO.
研究領(lǐng)域；細(xì)胞生物 免疫學(xué) 細(xì)菌及病毒
抗體來(lái)源；Rabbit
克隆類(lèi)型；Polyclonal
產(chǎn)品應(yīng)用；WB=1:500-2000
ELISA=1:5000-10000 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)）
not yet tested in
other applications.
optimal
dilutions/concentrations should be determined by the end user.
理論分子量；43/92kDa
細(xì)胞定位；細(xì)胞膜
性 狀；Liquid
濃 度；1mg/ml
免 疫 原；KLH conjugated synthetic
peptide derived from HIV2 gp36: 78-125/858
亞 型；IgG
純化方法；affinity purified by Protein A
緩 沖 液；0.01M TBS(pH7.4) with 1% BSA,
0.03% Proclin300 and 50% Glycerol.
保存條件；Shipped at 4℃. Store at -20 °C for one year. Avoid
repeated freeze/thaw cycles.
注意事項(xiàng)；This product as supplied is
intended for research use only, not for use in human, therapeutic or diagnostic
applications.
產(chǎn)品介紹 Human immunodeficiency virus
type 2 (HIV2), orginally isolated from patients in West Africa, is the dominant
form of HIV in West Africa capable of causing the acquired immunodeficiency
syndrome (AIDS). HIV2 is closely related to simian immunodeficiency viruses
(SIV). HIV1 and HIV2 share similarity in their genomes, transmission, clinical
features, immunological effects, and in their action of binding to the same CD4
cellular receptor, but there are significant differences in the amino acid and
nucleotide sequences of HIV1 and HIV2, especially within their envelope genes
and proteins. Additionally, HIV2 may have a longer incubation period and may be
less pathogenic than HIV1. HIV2 gp36 is a transmembrane protein located in the
envelope of the virus specific to HIV2 that binds to the putative cellular
receptor proteins P45 and P62.
The surface protein
gp120 (SU) attaches the virus to the host lymphoid cell by binding to the
primary receptor CD4. This interaction induces a structural rearrangement
creating a high affinity binding site for a chemokine coreceptor like CXCR4
and/or CCR5. This peculiar 2 stage receptor-interaction strategy allows gp120
to maintain the highly conserved coreceptor-binding site in a cryptic conformation,
protected from neutralizing antibodies. Since CD4 also displays a binding site
for the disulfide-isomerase P4HB/PDI, a P4HB/PDI-CD4-CXCR4-gp120 complex may
form. In that complex, P4HB/PDI could reach and reduce gp120 disulfide bonds,
causing major conformational changes in gp120. TXN, another PDI family member
could also be involved in disulfide rearrangements in Env during fusion. These
changes are transmitted to the transmembrane protein gp41 and are thought to
activate its fusogenic potential by unmasking its fusion peptide.
The surface protein gp120 is a ligand for CD209/DC-SIGN and CLEC4M/DC-SIGNR, which are respectively found on dendritic cells (DCs), and on endothelial cells of liver sinusoids and lymph node sinuses. These interactions allow capture of viral particles at mucosal surfaces by these cells and subsequent transmission to permissive cells. DCs are professional antigen presenting cells, critical for host immunity by inducing specific immune responses against a broad variety of pathogens. They act as sentinels in various tissues where they take up antigen, process it, and present it to T-cells following migration to lymphoid organs. HIV subverts the migration properties of dendritic cells to gain access to CD4+ T-cells in lymph nodes. Virus transmission to permissive T-cells occurs either in trans (without DCs infection, through viral capture and transmission), or in cis (following DCs productive infection, through the usual CD4-gp120 interaction), thereby inducing a robust infection. In trans infection, bound virions remain infectious over days and it is proposed that they are not degraded, but protected in non-lysosomal acidic organelles within the DCs close to the cell membrane thus contributing to the viral infectious potential during DCs' migration from the periphery to the lymphoid tissues. On arrival at lymphoid tissues, intact virions recycle back to DCs' cell surface allowing virus transmission to CD4+ T-cells. Virion capture also seems to lead to MHC-II-restricted viral antigen presentation, and probably to the activation of HIV-specific CD4+ cells.
實(shí)驗(yàn)流程：
（1）特異性結(jié)合抗原：抗體本身不能直接溶解或殺傷帶有特異抗原的靶細(xì)胞，通常需要補(bǔ)體或吞噬細(xì)胞等共同發(fā)揮效應(yīng)以清除病原微生物或?qū)е虏±頁(yè)p傷。然而，抗體可通過(guò)與病毒或毒素的特異性結(jié)合，直接發(fā)揮中和病毒的作用。
（2）活補(bǔ)體：IgM、IgG1、IgG2和IgG3可通過(guò)經(jīng)典途徑激活補(bǔ)體，凝聚的IgA、IgG4和IgE可通過(guò)替代途徑激活補(bǔ)體。
（3）結(jié)合細(xì)胞：不同類(lèi)別的免疫球蛋白，可結(jié)合不同種的細(xì)胞，參與免疫應(yīng)答。
（4）可通過(guò)胎盤(pán)及粘膜：免疫球蛋白G（IgG）能通過(guò)胎盤(pán)進(jìn)入胎兒血流中，使胎兒形成自然被動(dòng)
免疫。免疫球蛋白A（IgA）可通過(guò)消化道及呼吸道粘膜，是粘膜局部抗感染免疫的主要因素。
（5）具有抗原性：抗體分子是一種蛋白質(zhì)，也具有刺機(jī)體產(chǎn)生免疫應(yīng)答的性能。不同的免疫球蛋白分子，各具有不同的抗原性。
（6）抗體對(duì)理化因子的抵抗力與一般球蛋白相同：不耐熱，60～70℃即被破壞。各種酶及能使蛋白質(zhì)凝固變性的物質(zhì)，均能破壞抗體的作用?？贵w可被中性鹽類(lèi)沉淀。在生產(chǎn)上?？捎昧蛩徜@或硫酸鈉從免疫血清中沉淀出含有抗體的球蛋白，再經(jīng)透析法將其純化。
抗體的制備過(guò)程：
1.免疫原：普通的大分子蛋白，通過(guò)分子克隆構(gòu)建載體并在大腸桿菌中進(jìn)行誘導(dǎo)表達(dá)獲得重組蛋白，純化鑒定后可直接作為免疫原；小分子蛋白或化合物等分子量小，需要偶聯(lián)載體對(duì)該分子進(jìn)行改造才能使其成為具有免疫原性的抗原，常見(jiàn)偶聯(lián)載體如BSA、OVA、HAS等。
2. 免疫動(dòng)物：常用于制備抗血清的動(dòng)物有豚鼠、家兔、雞、大小鼠等，大量生產(chǎn)時(shí)需要用到狗、綿羊、山羊等。
3. 免疫血清的收集：一般家兔、綿羊、山羊可采用靜動(dòng)脈采血，家兔、豚鼠、大鼠、雞可采用心臟采血，家兔、山羊、綿羊可采用靜脈采血。
4. 免疫血清的純化與鑒定：得到的抗血清需要進(jìn)一步的純化，利用偶聯(lián)了抗原的親和柱進(jìn)行層析，具有高效，特異性強(qiáng)，純度高的特定。接著要鑒定純化蛋白的含量、相對(duì)分子的質(zhì)量、純度以及特異性。
5.人類(lèi)免疫缺陷病毒2型/2型艾滋病病毒gp36抗體免疫血清的保存：抗體一般比較穩(wěn)定，在-80℃ ～-20 ℃可以保存約5年而不會(huì)影響效價(jià)，而真空干燥保存時(shí)間可以更久。保存前需經(jīng)除菌并添加防腐劑。
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