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簡(jiǎn)要描述：丙型肝炎病毒E1抗體注意事項(xiàng)；This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Function: E1 and E2 glycoproteins form a heterodimer that is involved in virus attachment to the host cell, virion internalization through clathrin-dependent endocytosis and fusion with host membrane. E1/E2 heterodimer binds to human LDLR, CD81 and SCARB1/SR-BI receptors, but this binding is not sufficient for infection, some additional liver specific cofactors may be needed. The fusion function may possibly be carried by E1. E2 inhibits human EIF2AK2/PKR activation, preventing the establishment of an antiviral state. E2 is a viral ligand for CD209/DC-SIGN and CLEC4M/DC-SIGNR, which are respectively found on dendritic cells (DCs), and on liver sinusoidal endothelial cells and macrophage-like cells of lymph node sinuses. These interactions allow capture of circulating HCV particles by these cells and subsequent transmission to permissive cells. DCs act as sentinels in various tissues where they entrap pathogens and convey them to local lymphoid tissue or lymph node for establishment of immunity. Capture of circulating HCV particles by these SIGN+ cells may facilitate virus infection of proximal hepatocytes and lymphocyte subpopulations and may be essential for the establishment of persistent infection.
產(chǎn)品型號(hào)：GOY-01K5055廠商性質(zhì)：科研更新時(shí)間：2023-10-24 00:00:00訪 問(wèn) 量：135產(chǎn)品分類(lèi)
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商品屬性：
貨號(hào) | 產(chǎn)品名稱(chēng) | 規(guī)格 |
GOY-01K5055 | 丙型肝炎病毒E1抗體 | 50ul |
GOY-01K5055 | 丙型肝炎病毒E1抗體 | 100ul |
GOY-01K5055 | 丙型肝炎病毒E1抗體 | 200ul |
英文名稱(chēng): Hepatitis C virus subtype 1b E1
中文名稱(chēng): 丙型肝炎病毒E1抗體
別 名；Hepatitis C Virus E1; E1 protein; Envelope
glycoprotein E1; Gp32; Gp35; HCV E1; HCV envelope glycoprotein E1; Hepatitis C
virus envelope glycoprotein E1.
研究領(lǐng)域；細(xì)菌及病毒
抗體來(lái)源；Rabbit
克隆類(lèi)型；Polyclonal
產(chǎn)品應(yīng)用；ELISA=1:5000-10000
not yet tested in
other applications.
optimal
dilutions/concentrations should be determined by the end user.
理論分子量；21kDa
性 狀；Liquid
濃 度；1mg/ml
免 疫 原；KLH conjugated synthetic
peptide derived from Hepatitis C Virus E1: 201-300/3010
亞 型；IgG
純化方法；affinity purified by Protein A
緩 沖 液；0.01M TBS(pH7.4) with 1% BSA,
0.03% Proclin300 and 50% Glycerol.
保存條件；Shipped at 4℃. Store at -20 °C for one year. Avoid
repeated freeze/thaw cycles.
注意事項(xiàng)；This product as supplied is
intended for research use only, not for use in human, therapeutic or diagnostic
applications.
Function:
E1 and E2
glycoproteins form a heterodimer that is involved in virus attachment to the
host cell, virion internalization through clathrin-dependent endocytosis and
fusion with host membrane. E1/E2 heterodimer binds to human LDLR, CD81 and
SCARB1/SR-BI receptors, but this binding is not sufficient for infection, some
additional liver specific cofactors may be needed. The fusion function may
possibly be carried by E1. E2 inhibits human EIF2AK2/PKR activation, preventing
the establishment of an antiviral state. E2 is a viral ligand for CD209/DC-SIGN
and CLEC4M/DC-SIGNR, which are respectively found on dendritic cells (DCs), and
on liver sinusoidal endothelial cells and macrophage-like cells of lymph node
sinuses. These interactions allow capture of circulating HCV particles by these
cells and subsequent transmission to permissive cells. DCs act as sentinels in
various tissues where they entrap pathogens and convey them to local lymphoid
tissue or lymph node for establishment of immunity. Capture of circulating HCV
particles by these SIGN+ cells may facilitate virus infection of proximal
hepatocytes and lymphocyte subpopulations and may be essential for the
establishment of persistent infection.
實(shí)驗(yàn)流程：
（1）特異性結(jié)合抗原：抗體本身不能直接溶解或殺傷帶有特異抗原的靶細(xì)胞，通常需要補(bǔ)體或吞噬細(xì)胞等共同發(fā)揮效應(yīng)以清除病原微生物或?qū)е虏±頁(yè)p傷。然而，抗體可通過(guò)與病毒或毒素的特異性結(jié)合，直接發(fā)揮中和病毒的作用。
（2）活補(bǔ)體：IgM、IgG1、IgG2和IgG3可通過(guò)經(jīng)典途徑激活補(bǔ)體，凝聚的IgA、IgG4和IgE可通過(guò)替代途徑激活補(bǔ)體。
（3）結(jié)合細(xì)胞：不同類(lèi)別的免疫球蛋白，可結(jié)合不同種的細(xì)胞，參與免疫應(yīng)答。
（4）可通過(guò)胎盤(pán)及粘膜：免疫球蛋白G（IgG）能通過(guò)胎盤(pán)進(jìn)入胎兒血流中，使胎兒形成自然被動(dòng)
免疫。免疫球蛋白A（IgA）可通過(guò)消化道及呼吸道粘膜，是粘膜局部抗感染免疫的主要因素。
（5）具有抗原性：抗體分子是一種蛋白質(zhì)，也具有刺機(jī)體產(chǎn)生免疫應(yīng)答的性能。不同的免疫球蛋白分子，各具有不同的抗原性。
（6）抗體對(duì)理化因子的抵抗力與一般球蛋白相同：不耐熱，60～70℃即被破壞。各種酶及能使蛋白質(zhì)凝固變性的物質(zhì)，均能破壞抗體的作用?？贵w可被中性鹽類(lèi)沉淀。在生產(chǎn)上常可用硫酸銨或硫酸鈉從免疫血清中沉淀出含有抗體的球蛋白，再經(jīng)透析法將其純化。
抗體的制備過(guò)程：
1.丙型肝炎病毒E1抗體免疫原：普通的大分子蛋白，通過(guò)分子克隆構(gòu)建載體并在大腸桿菌中進(jìn)行誘導(dǎo)表達(dá)獲得重組蛋白，純化鑒定后可直接作為免疫原；小分子蛋白或化合物等分子量小，需要偶聯(lián)載體對(duì)該分子進(jìn)行改造才能使其成為具有免疫原性的抗原，常見(jiàn)偶聯(lián)載體如BSA、OVA、HAS等。
2. 免疫動(dòng)物：常用于制備抗血清的動(dòng)物有豚鼠、家兔、雞、大小鼠等，大量生產(chǎn)時(shí)需要用到狗、綿羊、山羊等。
3. 免疫血清的收集：一般家兔、綿羊、山羊可采用靜動(dòng)脈采血，家兔、豚鼠、大鼠、雞可采用心臟采血，家兔、山羊、綿羊可采用靜脈采血。
4. 免疫血清的純化與鑒定：得到的抗血清需要進(jìn)一步的純化，利用偶聯(lián)了抗原的親和柱進(jìn)行層析，具有高效，特異性強(qiáng)，純度高的特定。接著要鑒定純化蛋白的含量、相對(duì)分子的質(zhì)量、純度以及特異性。
5. 免疫血清的保存：抗體一般比較穩(wěn)定，在-80℃ ～-20 ℃可以保存約5年而不會(huì)影響效價(jià)，而真空干燥保存時(shí)間可以更久。保存前需經(jīng)除菌并添加防腐劑。
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